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Human M-CSF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Human M-CSF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

M-CSF Recombinant Protein

CATALOG NUMBER: 96-923

Tested Applications:
ELISA, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Glu 33 - Arg 255
Fusion Tag:
His Tag
Tested Applications:
ELISA, WB
Application Note:
This recombinant protein can be used for E, WB. For research use only.
Predicted Molecular Weight:
26 kDa
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human M-CSF, His Tag at 5 ug/mL (100 uL/well) can bind Human M-CSF R, Fc Tag with a linear range of 0.17-1.52 ng/mL.
Purity:
>95% as determined by SDS-PAGE.

Endotoxin level is less than 1.0 EU per ug by the LAL method.
physical-state:
Lyophilized
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
M-CSF
Additional Names:
M-CSF,CSF-1,Lanimostim
Protein Accession Number:
P09603-1
Ncbi Gene Id Number:
1435
Background:
The colony stimulating factor 1 (CSF1), also known as macrophage colony-stimulating factor (M-CSF), is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. Eukaryotic cells also produce M-CSF in order to combat intercellular viral infection. It is one of the three experimentally described colony-stimulating factors. M-CSF binds to the colony stimulating factor 1 receptor. Macrophage colony-stimulating factor has been shown to interact with PIK3R2. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. Locally produced M-CSF in the vessel wall contributes to the development and progression of atherosclerosis.
Background Reference 1:
Sapi E., 2004, Exp. Biol. Med., 229 : 1-11.
Background Reference 2:
Jang Mei-Huei, 2006, The Journal of Immunology, 177 (6): 4055-4063.
Background Reference 3:
Cao, Qi, 2014, Kidney International, 85 (4): 794-806.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-923

List Size:
0.05 mg

List Price:

$577.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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