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Western Blot at 1:1000 dilution + U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of mouse anti-Semaphorin 5A Pab in mouse brain tissue lysates (35ug/lane).
Western Blot at 1:1000 dilution + U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.

M Sema5a Antibody

CATALOG NUMBER: 61-441

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This Mouse Sema5a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1006-1035 amino acids from mouse Sema5a.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
121 kDa
Purification:
This antibody is purified through a protein G column, followed by dialysis against PBS.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SEMA5A
Additional Names:
Semaphorin-5A, Semaphorin-F, Sema F, SEMA5A, SEMAF
Protein Accession Number:
Q13591
Protein Gi Number:
109939725
Ncbi Gene Id Number:
9037
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Members of the semaphorin protein family, such as SEMA5A, are involved in axonal guidance during neural development.
Background Reference 1:
Bialecka,M., Neurosci. Lett. 399 (1-2), 121-123 (2006)
Background Reference 2:
Melin,M., Neuropsychobiology 54 (1), 64-69 (2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-441

List Size:
400 ul

List Price:

$495.00

Datasheet
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