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Western Blot at 1:64000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + CHO whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of lysate from mouse heart tissue lysate, using Mouse Sirt3 Antibody at 1:1000.
Western blot analysis of Sirt3 Antibody in mouse brain tissue lysates (35ug/lane)
Western blot analysis of Sirt3 Antibody in CHO cell line lysates (35ug/lane)
Sirt3 Antibody IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:64000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane.

M Sirt3 Antibody

CATALOG NUMBER: 62-194

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Hamster, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Hamster, Mouse
Immunogen:
This Sirt3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-334 amino acids from the C-terminal region of Mouse Sirt3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
37 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Sirt3
Additional Names:
NAD-dependent protein deacetylase sirtuin-3, 351-, Regulatory protein SIR2 homolog 3, SIR2-like protein 3, mSIR2L3, Sirt3, Sir2l3
Protein Accession Number:
Q8R104
Ncbi Gene Id Number:
64384
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.
Background Reference 1:
Hirschey, M.D., et al. Nature 464(7285):121-125(2010)
Background Reference 2:
Pillai, V.B., et al. J. Biol. Chem. 285(5):3133-3144(2010)
Background Reference 3:
Kim, H.S., et al. Cancer Cell 17(1):41-52(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-194

List Size:
200 ul

List Price:

$495.00

Datasheet
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