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Western blot analysis of M6PR Antibody in mouse brain tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with M6PR antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of M6PR Antibody in mouse brain tissue lysates (35ug/lane)

M6PR Antibody

CATALOG NUMBER: 61-508

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This M6PR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-63 amino acids from the N-terminal region of human M6PR.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
31 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
M6PR
Additional Names:
Cation-dependent mannose-6-phosphate receptor, CD Man-6-P receptor, CD-MPR, 46 kDa mannose 6-phosphate receptor, MPR 46, M6PR, MPR46, MPRD
Protein Accession Number:
P20645
Protein Gi Number:
127293
Ncbi Gene Id Number:
4074
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
M6PR is a receptor for mannose-6-phosphate groups on lysosomal enzymes. The receptor forms a homodimer or homotetramer for intracellular targeting of lysosomal enzymes and export of newly synthesized lysosomal enzymes into the cell secretions. The receptor is an integral membrane protein which localizes to the trans-Golgi reticulum, endosomes, and the plasma membrane.
Background Reference 1:
Perez-Victoria,F.J., Mol. Biol. Cell 19 (6), 2350-2362 (2008)
Background Reference 2:
Mari,M., Traffic(3), 380-393 (2008)
Background Reference 3:
Lemansky,P., J. Leukoc. Biol. 81 (4), 1149-1158 (2007)
Background Reference 4:
Chen,J.J., Cell 119 (7), 915-926 (2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-508

List Size:
400 ul

List Price:

$495.00

Datasheet
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