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Western blot analysis in A549 cell line lysates (35ug/lane).
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of M6PR Antibody (AP2792b) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis in A549 cell line lysates (35ug/lane).

M6PR Antibody

CATALOG NUMBER: 61-509

Clonality:
Polyclonal
Tested Applications:
Flow, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This M6PR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-260 amino acids from the C-terminal region of human M6PR.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
31 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
M6PR
Additional Names:
Cation-dependent mannose-6-phosphate receptor, CD Man-6-P receptor, CD-MPR, 46 kDa mannose 6-phosphate receptor, MPR 46, M6PR, MPR46, MPRD
Protein Accession Number:
P20645
Protein Gi Number:
127293
Ncbi Gene Id Number:
4074
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a receptor formannose-6-phosphate groups on lysosomal enzymes. The receptor formsa homodimer or homotetramer for intracellular targeting oflysosomal enzymes and export of newly synthesized lysosomal enzymesinto the cell secretions. The receptor is an integral membraneprotein which localizes to the trans-Golgi reticulum, endosomes,and the plasma membrane.
Background Reference 1:
Bosker, F.J., et al. Mol. Psychiatry (2010) In press :
Background Reference 2:
Op den Brouw, M.L., et al. Virology 393(1):84-90(2009)
Background Reference 3:
Schimanski, L.M., et al. Blood Cells Mol. Dis. 43(2):180-193(2009)
Background Reference 4:
Rodriguez-Gabin, A.G., et al. Exp. Cell Res. 315(13):2215-2230(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-509

List Size:
400 ul

List Price:

$495.00

Datasheet
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