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MAD3 Antibody

CATALOG NUMBER: 45-854

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-QEHSYSHGGGAWL
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:No signal obtained yet but low background observed in NCI-H460, HepG2, Human Liver, Human Heart and Human Brain lysates at up to 3ug/ml.Immunohistochemistry:An anonymous customer found positive results in IHC on Human HeLa cells.Immunofluorescence: This product has been successfully used by an anonymous customer on methanol-fixed and 0.5% TX100-permeabilized HeLa cells, staning the cytoplasm.
Specificity:
This antibody is expected to recognise isoform a (NP_112590.1) only.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MXD3
Additional Names:
MXD3, MAD3, MAX dimerization protein 3, MGC2383, hypothetical protein MGC2383, likely ortholog of mouse Max dimerization protein 3, BHLHC13, FLJ35523, MYX, Max-associated protein 3, Max-interacting transcriptional repressor MAD3
Protein Accession Number:
NP_112590.1
Protein Gi Number:
13775222
Ncbi Gene Id Number:
83463
Background Reference 1:
Hurlin PJ, Queva C, Koskinen PJ, Steingrimsson E, Ayer DE, Copeland NG, Jenkins NA, Eisenman RN. Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation. EMBO J. 1995 Nov 15;14(22):5646-59.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-854

List Size:
0.1 mg

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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