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Western blot analysis of Maelstrom in HeLa cell lysate with Maelstrom antibody at 1 μg/mL.
Immunocytochemistry of Maelstrom in HeLa cells with Maelstrom antibody at 5 μg/mL.
Immunofluorescence of Maelstrom in HeLa cells with Maelstrom antibody at 20 μg/mL.
Western blot analysis of Maelstrom in HeLa cell lysate with Maelstrom antibody at 1 μg/mL.
Trial Size

Maelstrom Antibody

CATALOG NUMBER: 5667

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%), Pig: (88%)
Immunogen:
Maelstrom antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human Maelstrom.
The immunogen is located within amino acids 30 - 80 of Maelstrom.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
Maelstrom antibody can be used for detection of Maelstrom by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Purification:
Maelstrom Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Maelstrom Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Maelstrom antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAEL
Additional Names:
Maelstrom Antibody: CT128, SPATA35, Protein maelstrom homolog
Protein Accession Number:
AAY82463
Protein Gi Number:
67866977
Ncbi Gene Id Number:
84944
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Maelstrom Antibody: The mammalian homolog of the Drosophila protein Maelstrom is expressed in the male germline and localizes to the sex body in spermatocytes and the chromatoid body in round spermatids. Similar to its expression in Drosophila, Maelstrom is a component of nuages, a germ-cell specific organelle and is thought to be essential for spermatogenesis and transposon repression during meiosis. In humans, Maelstrom has been found to be expressed only in the testis and in various cancer cell lines. Treatment of these cell lines with the demethylating agent 5'-Aza-2-Deoxycytidine significantly upregulated Maelstrom levels, indicating that its expression is regulated by DNA methylation.
Background Reference 1:
Clegg NJ, Frost DM, Larkin MK, et al. Maelstrom is required for an early step in the establishment of Drosophila oocyte polarity: posterior localization of grk mRNA. Development1997; 124:4661-71.
Background Reference 2:
Costa Y, Speed RM, Gautier P, et al. Mouse MAELSTROM: the link between meiotic silencing of unsynapsed chromatin and microRNA pathway? Hum. Mol. Genet.2006; 15:2324-34.
Background Reference 3:
Soper SFC, van der Heijen GW, Hardiman TC, et al. Mouse Maelstrom, a component of nuage, is essential for spermatogenesis and transposon repression in meiosis. Dev. Cell2008; 15:285-97.
Background Reference 4:
Xiao L, Wang Y, Zhou Y, et al. Identification of a novel human/testis gene MAEL that is regulated by DNA methylation. Mol. Biol. Rep.2009; epub.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5667

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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