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Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
MAF1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).

MAF1 Antibody

CATALOG NUMBER: 55-086

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This MAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-117 amino acids from the Central region of human MAF1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
29 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAF1
Additional Names:
Repressor of RNA polymerase III transcription MAF1 homolog, MAF1
Protein Accession Number:
Q9H063
Protein Gi Number:
296434582
Ncbi Gene Id Number:
84232
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that is similar to Maf1, aSaccharomyces cerevisiae protein highly conserved in eukaryoticcells. Yeast Maf1 is a negative effector of RNA polymerase III (PolIII). It responds to changes in the cellular environment andrepresses pol III transcription. Biochemical studies identified theinitiation factor TFIIIB as a target for Maf1-dependent repression.
Background Reference 1:
Kantidakis, T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(26):11823-11828(2010)
Background Reference 2:
Shor, B., et al. J. Biol. Chem. 285(20):15380-15392(2010)
Background Reference 3:
Johnson, S.S., et al. Mol. Cell 26(3):367-379(2007)
Background Reference 4:
Lamesch, P., et al. Genomics 89(3):307-315(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-086

List Size:
400 ul

List Price:

$495.00

Datasheet
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