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Western blot analysis in K562 cell line lysates (35ug/lane).
Western blot analysis in K562 cell line lysates (35ug/lane).

MAFA Antibody

CATALOG NUMBER: 58-295

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This MAFA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the C-terminal region of human MAFA.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
37 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAFA
Additional Names:
Transcription factor MafA, Pancreatic beta-cell-specific transcriptional activator, Transcription factor RIPE3b1, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, MAFA
Protein Accession Number:
Q8NHW3
Protein Gi Number:
296435511
Ncbi Gene Id Number:
389692
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MAFA is a transcription factor that binds RIPE3b, aconserved enhancer element that regulates pancreatic betacell-specific expression of the insulin gene (INS; MIM 176730)(Olbrot et al., 2002 [PubMed 12011435]).
Background Reference 1:
Lecoin, L., et al. Dev Neurobiol 70(7):485-497(2010)
Background Reference 2:
Guo, S., et al. J. Biol. Chem. 285(17):12655-12661(2010)
Background Reference 3:
Kondo, T., et al. Mol. Endocrinol. 23(8):1281-1290(2009)
Background Reference 4:
Kaneto, H., et al. Adv. Drug Deliv. Rev. 61 (7-8), 489-496 (2009) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-295

List Size:
400 ul

List Price:

$495.00

Datasheet
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