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Western blot analysis of extracts of 22Rv1 cells, using MAGEA1 antibody (19-684) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human breast cancer using MAGEA1 antibody (19-684) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using MAGEA1 antibody (19-684) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach cancer using MAGEA1 antibody (19-684) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using MAGEA1 antibody (19-684). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of 22Rv1 cells, using MAGEA1 antibody (19-684) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

MAGEA1 Antibody

CATALOG NUMBER: 19-684

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human MAGEA1 (NP_004979.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Positive Control 1:
22Rv1
Predicted Molecular Weight:
Observed: 45kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MAGEA1
Additional Names:
MAGEA1, MAGE1, MGC9326, CT1.1
Ncbi Gene Id Number:
4100
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-684

List Size:
100 uL

List Price:

$436.00

Datasheet
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