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Western blot analysis of anti-MAGEA11 Antibody pre-incubated with and without blocking peptide in 293 cell line lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of anti-MAGEA11 Antibody pre-incubated with and without blocking peptide in 293 cell line lysate.

MAGEA11 Antibody

CATALOG NUMBER: 62-150

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MAGEA11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-228 amino acids from the N-terminal region of human MAGEA11.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
48 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAGEA11
Additional Names:
Melanoma-associated antigen 11, Cancer/testis antigen 111, CT111, MAGE-11 antigen, MAGEA11, MAGE11
Protein Accession Number:
P43364
Protein Gi Number:
97536722
Ncbi Gene Id Number:
4110
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MAGEA11 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80% sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are expressed at a high level in a number of tumors of various histologic types, and are silent in normal tissues with the exception of testis and placenta. The MAGEA genes are clustered on chromosome Xq28. They may be implicated in some hereditary disorders, such as dyskeratosis congenita.
Background Reference 1:
Rogner, U.C., et al., Genomics 29(3):725-731 (1995).
Background Reference 2:
De Plaen, E., et al., Immunogenetics 40(5):360-369 (1994).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-150

List Size:
400 ul

List Price:

$495.00

Datasheet
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