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Western blot analysis of MAGEA4 in A431 cell lysate with MAGEA4 antibody at 1 μg/ml.
Immunohistochemistry of MAGEA4 in human breast tumor with MAGEA4 antibody at 5 μg/mL.
Immunofluorescence of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 μg/ml.
Immunofluorescence of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 μg/ml.<br><br>Green: MAGEA4 Antibody (8191) <br> Blue: DAPI staining
Immunocytochemistry of MAGEA4 in HeLa cells with MAGEA4 antibody at 5 μg/ml.
Immunofluorescence of MAGEA4 inHeLa cells with MAGEA4 antibody at 20 μg/ml.<br><br>Red: MAGEA4 Antibody (8191) <br> Blue: DAPI staining
Western blot analysis of MAGEA4 in A431 cell lysate with MAGEA4 antibody at 1 μg/ml.
Trial Size

MAGEA4 Antibody

CATALOG NUMBER: 8191

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
MAGEA4 antibody was raised against a 16 amino acid peptide near the amino terminus of human MAGEA4.
The immunogen is located within the first 50 amino acids of MAGEA4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
MAGEA4 antibody can be used for detection of MAGEA4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
MAGEA4 antibody is human specific. At least four isoforms of MAGEA4 are known to exist.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Positive Control 2:
Cat. No. 10-001 - Human Breast Tissue Slide
Predicted Molecular Weight:
Predicted: 35 kDa
Observed: 45 kDa
Purification:
MAGEA4 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MAGEA4 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MAGEA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
MAGEA4
Additional Names:
Melanoma antigen family A4, MAGE4, MAGE-41, MAGE4A, MAGE-4 antigen, MAGE4B, MAGE-X2, MAGE-X2 antigen, Melanoma-associated antigen 4
Protein Accession Number:
NP_001011550
Protein Gi Number:
58530871
Ncbi Gene Id Number:
4103
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MAGEA4 is a member of the melanoma-associated antigen family that consists of a number of antigens recognized by cytotoxic T lymphocytes (1). MAGEA4 may serve as a target for antitumoral vaccination and may play a role in embryonal development and tumor transformation or aspects of tumor progression. It is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma and have been implicated in some hereditary disorders, such as dyskeratosis congenital (2-4).
Background Reference 1:
Bhan S, Negi SS, Shao C, et al. BORIS binding to the promoters of cancer testis antigens, MAGEA2, MAGEA3, and MAGEA4, is associated with their transcriptional activation in lung cancer. Clin. Cancer Res. 2011; 17:4267-76.
Background Reference 2:
Bhan S, Chuang A, Negi SS, et al. MAGEA4 induces growth in normal oral keratinocytes by inhibiting growth arrest and apoptosis. Oncol. Rep. 2012; 28:1498-502.
Background Reference 3:
Saito T, Wada H, Yamasaki M, et al. High expression of MAGE-A4 and MHC class I antigens in tumor cells and induction of MAGE-A4 immune responses are prognostic markers of CHP-MAGE-A4 cancer vaccine. Vaccine, epub. 2014; S0264-410X(14)01217-1.
Background Reference 4:
Zhang QM, He SJ, Shen N, et al. Overexpression of MAGE-D4 in colorectal cancer is a potentially prognostic biomarker and immunotherapy target. Int. J. Clin. Exp. Pathol. 2014; 7:3918-27.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8191

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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