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Western blot analysis of MAGEC1 antibody in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of MAGEC1 antibody in K562 cell line lysates (35ug/lane)

MAGEC1 Antibody

CATALOG NUMBER: 62-156

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MAGEC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 849-880 amino acids from the C-terminal region of human MAGEC1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
124 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAGEC1
Additional Names:
Melanoma-associated antigen C1, Cancer/testis antigen 71, CT71, MAGE-C1 antigen, MAGEC1
Protein Accession Number:
O60732
Ncbi Gene Id Number:
9947
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MAGEC1 is a member of the melanoma antigen gene (MAGE) family. The proteins of this family are tumor-specific antigens that can be recognized by autologous cytolytic T lymphocytes. This protein contains a large number of unique short repetitive sequences in front of the MAGE-homologous sequence, thus is about 800 residues longer than the other MAGE proteins. This gene is expressed in a significant proportion of tumors of various histological types, whereas it is silent in normal tissues, except testis. The function of this gene is unknown.
Background Reference 1:
Jungbluth, A.A., et al., Int. J. Cancer 99(6):839-845 (2002).
Background Reference 2:
Chen, Y.T., et al., Proc. Natl. Acad. Sci. U.S.A. 95(12):6919-6923 (1998).
Background Reference 3:
Lucas, S., et al., Cancer Res. 58(4):743-752 (1998).
Background Reference 4:
Lim, S.H., et al., Eur. J. Haematol. 67 (5-6), 332-334 (2001).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-156

List Size:
400 ul

List Price:

$495.00

Datasheet
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