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Antibody is used in Western blot to detect MAGED1 in A549 lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Antibody is used in Western blot to detect MAGED1 in A549 lysate.

MAGED1 Antibody

CATALOG NUMBER: 62-157

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Pig
Immunogen:
This MAGED1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 426-456 amino acids from the Central region of human MAGED1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
86 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAGED1
Additional Names:
Melanoma-associated antigen D1, MAGE tumor antigen CCF, MAGE-D1 antigen, Neurotrophin receptor-interacting MAGE homolog, MAGED1, NRAGE
Protein Accession Number:
Q9Y5V3
Protein Gi Number:
62906893
Ncbi Gene Id Number:
9500
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MAGED1 is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway.
Background Reference 1:
Sasaki, A., et al., J. Biol. Chem. 277(25):22541-22546 (2002).
Background Reference 2:
Kubu, C.J., et al., Genomics 70(1):150-152 (2000).
Background Reference 3:
Salehi, A.H., et al., Neuron 27(2):279-288 (2000).
Background Reference 4:
Pold, M., et al., Genomics 59(2):161-167 (1999).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-157

List Size:
400 ul

List Price:

$495.00

Datasheet
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