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Antibody is used in Western blot to detect MAGED2 in A549 lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Antibody is used in Western blot to detect MAGED2 in A549 lysate.

MAGED2 Antibody

CATALOG NUMBER: 62-158

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MAGED2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 576-606 amino acids from the C-terminal region of human MAGED2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
65 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAGED2
Additional Names:
Melanoma-associated antigen D2, 11B6, Breast cancer-associated gene 1 protein, BCG-1, Hepatocellular carcinoma-associated protein JCL-1, MAGE-D2 antigen, MAGED2, BCG1
Protein Accession Number:
Q9UNF1
Protein Gi Number:
17380153
Ncbi Gene Id Number:
10916
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MAGED2 is a member of the MAGED gene family. In contrast to the MAGEA and MAGEB genes, which have their entire coding sequences located in the last exon, the MAGED genes have their coding sequences located throughout multiple exons. Also, the MAGEA and MAGEB genes are silent in normal tissues with the exception of testis and placenta, whereas the MAGED genes are expressed ubiquitously. The MAGED genes are clustered on chromosome Xp11. This gene is located in Xp11.2, a hot spot for X-linked mental retardation (XLMR).
Background Reference 1:
Lucas, S., et al., Cancer Res. 59(16):4100-4103 (1999).
Background Reference 2:
Langnaese, K., et al., Cytogenet. Cell Genet. 94 (3-4), 233-240 (2001).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-158

List Size:
400 ul

List Price:

$495.00

Datasheet
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