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Antibody is used in Western blot to detect MAGEH1 in Jurkat cell lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of MAGEH1 using rabbit polyclonal MAGEH1 Antibody (D201) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAGEH1 gene.
Antibody is used in Western blot to detect MAGEH1 in Jurkat cell lysate.

MAGEH1 Antibody

CATALOG NUMBER: 62-160

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This MAGEH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-218 amino acids from the C-terminal region of human MAGEH1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
24 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAGEH1
Additional Names:
Melanoma-associated antigen H1, Apoptosis-related protein 1, APR-1, MAGE-H1 antigen, Restin, MAGEH1, APR1
Protein Accession Number:
Q9H213
Protein Gi Number:
14423761
Ncbi Gene Id Number:
28986
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Melanoma-associated antigen (MAGE) are completely silent in normal tissues, with the exception of male germ cells, and, for some of them, placenta. These antigens ought to be strictly tumor specific, expressed in tumor cells of various histological types. Because of their specific expression on tumor cells, these antigens are of particular interest for antitumor immunotherapy. Genes of the MAGE family direct the expression of tumor antigens that are recognized on a human melanoma by autologous cytolytic T lymphocytes. Though the function of MAGEH1 is not fully elucidated, it may play a role in embryonal development and tumor transformation or aspects of tumor progression.
Background Reference 1:
Chomez, P., et al., Cancer Res. 61(14):5544-5551 (2001).
Background Reference 2:
Zhu, F., et al., BioTechniques 29(2):310-313 (2000).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-160

List Size:
400 ul

List Price:

$495.00

Datasheet
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