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Western blot analysis of MAML3 antibody in Hela cell line lysates (35ug/lane)
MAML3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lymphnode followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of MAML3 antibody in Hela cell line lysates (35ug/lane)

MAML3 Antibody

CATALOG NUMBER: 62-427

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MAML3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 640-665 amino acids from the C-terminal region of human MAML3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
122 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAML3
Additional Names:
Mastermind-like protein 3, Mam-3, MAML3 (HGNC:16272)
Protein Accession Number:
Q96JK9
Ncbi Gene Id Number:
55534
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MAML3 acts as a transcriptional coactivator for NOTCH proteins. The protein has been shown to amplify NOTCH-induced transcription of HES1.
Background Reference 1:
Tonon,G., Nat. Genet. 33 (2), 208-213 (2003)
Background Reference 2:
Lin,S.E., J. Biol. Chem. 277 (52), 50612-50620 (2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-427

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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