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45-859 (4ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-859 (4ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Mammaglobin A Antibody

CATALOG NUMBER: 45-859

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-NPQVSKTEYKEL
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as small as the calculated size of 10.5kDa according to NP_002402.1. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Breast shows staining of cytoplasm in some acinar cells.. Recommended concentration, 4-6ug/ml.
Positive Control 1:
Cat. No. 1311 - Human Breast Tissue Lysate
Positive Control 2:
Cat. No. 10-001 - Human Breast Tissue Slide
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SCGB2A2
Additional Names:
SCGB2A2, secretoglobin, family 2A, member 2 , HGNC:7050, MGB1, UGB2, MGC71974, mammaglobin 1, mammaglobin A
Protein Accession Number:
NP_002402.1
Protein Gi Number:
4505169
Ncbi Gene Id Number:
4250
Background Reference 1:
Ouellette RJ, Richard D, Maicas E. RT-PCR for mammaglobin genes, MGB1 and MGB2, identifies breast cancer micrometastases in sentinel lymph nodes. Am J Clin Pathol. 2004 May;121(5):637-43. Erratum in: Am J Clin Pathol. 2004 Nov;122(5):813.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-859

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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