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Western blot analysis of MAP1LC3A in 293 cell lysate with MAP1LC3A antibody at 1 μg/ml.
Immunohistochemistry of MAP1LC3A in human brain tissue with MAP1LC3A antibody at 5 μg/mL.
Immunofluorescence of MAP1LC3A in human brain tissue with MAP1LC3A antibody at 20 μg/mL.
Western blot analysis of MAP1LC3A in 293 cell lysate with MAP1LC3A antibody at 1 μg/ml.
Trial Size

MAP1LC3A Antibody

CATALOG NUMBER: 7579

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
MAP1LC3A antibody was raised against a 10 amino acid peptide near the amino terminus of human MAP1LC3A.
The immunogen is located within the first 50 amino acids of MAP1LC3A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
MAP1LC3A antibody can be used for detection of MAP1LC3A by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
MAP1LC3A antibody is human specific. At least two isoforms of MAP1LC3A are known to exist. MAP1LC3A antibody is predicted to not cross-react with MAP1LC3B or MAP1LC3C.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 3:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 14 kDa
Observed: 17 kDa
Purification:
MAP1LC3A antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MAP1LC3A antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MAP1LC3A antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
MAP1LC3A
Additional Names:
MAP1LC3A Antibody: LC3, LC3A, ATG8E, MAP1ALC3, MAP1BLC3Autophagy-related protein LC3 A
Protein Accession Number:
NP_852610
Protein Gi Number:
31563518
Ncbi Gene Id Number:
84557
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3A belongs to the MAP1 LC3 family of proteins that form mature complexes with MAP1A and MAP1B which are thought to be important in the formation and development of axons and dendrites (2). MAP1LC3A is one of three isoforms of MAP1LC3, the mammalian homolog of yeast ATG8, an essential autophagy protein. These isoforms exhibit distinct expression patterns and MAP1LC3A, like MAP1LC3A but not MAP1LC3B, is post-translationally modified, suggesting the three isoforms may have different physiological functions (3).
Background Reference 1:
Mandelkow E and Mandelkow EM. Microtubules and microtubule-associated proteins. Curr. Opin. Cell Biol. 1995; 7:72-81.
Background Reference 2:
Halpain S and Dehmelt L. The MAP1 family of microtubule-associated proteins. Genome Biol. 2006; 7:224.
Background Reference 3:
He H, Dang Y, Dai F, et al. Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B. J. Biol. Chem. 2003; 278:29278-87.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7579

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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