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Western blot analysis of extracts of various cell lines, using MAP1LC3A antibody (14-019).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse brain using MAP1LC3A antibody (14-019) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using MAP1LC3A antibody (14-019) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using MAP1LC3A antibody (14-019) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using MAP1LC3A antibody (14-019).
Immunofluorescence analysis of PC-12 cells using MAP1LC3A antibody (14-019).
Immunofluorescence analysis of HUVEC cells using MAP1LC3A antibody (14-019).
Western blot analysis of extracts of various cell lines, using MAP1LC3A antibody (14-019).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

MAP1LC3A Antibody [E2F7]

CATALOG NUMBER: 14-019

Clonality:
Monoclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human MAP1LC3A
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
SHG-44
Positive Control 2:
Mouse brain
Positive Control 3:
Mouse liver
Positive Control 4:
Mouse skeletal muscle
Predicted Molecular Weight:
Observed: 14kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MAP1LC3A
Additional Names:
MAP1LC3A Antibody: LC3, LC3A, ATG8E, MAP1ALC3, MAP1BLC3Autophagy-related protein LC3 A
Ncbi Gene Id Number:
84557
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-019

List Size:
50 uL

List Price:

$335.00

Out of stock

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