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Western blot analysis of lysates from SH-SY5Y cells using MAP1LC3A Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.
Western blot analysis of lysates from NIH/3T3,C6 cells using MAP1LC3A Rabbit mAb at 1:1000 dilution. NIH/3T3 cells and C6 cells were treated with Chloroquine (50 μM) at 37℃ for 20 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 180s.
Immunoprecipitation analysis of 300 μg extracts of NIH/3T3 cells using 3 μg MAP1LC3A antibody. Western blot was performed from the immunoprecipitate using MAP1LC3A antibody at a dilution of 1:1000.NIH/3T3 cells were treated with Chloroquine (50 μM) at 37℃ for 20 hours.
Western blot analysis of lysates from SH-SY5Y cells using MAP1LC3A Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.

MAP1LC3A Rabbit Recombinant Antibody (81-235)

CATALOG NUMBER: 81-235

Clonality:
Monoclonal
Tested Applications:
ELISA, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IP, WB
Application Note:
WB,1:1000 - 1:2000, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
NIH/3T3 treated with Chloroquine,C6 treated with Chloroquine,SH-SY5Y
Predicted Molecular Weight:
14kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MAP1LC3A
Additional Names:
LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3; MAP1LC3A
Protein Accession Number:
Q9H492
Ncbi Gene Id Number:
84557
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

81-235

List Size:
100 uL

List Price:

$477.00

Datasheet
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