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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of MAP2K2-S222 in Y79 cell line lysates (35ug/lane)
Western blot analysis of MAP2K2 using rabbit polyclonal MAP2K2-S222 using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2).
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP2K2 Antibody (S222) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.

MAP2K2 Antibody

CATALOG NUMBER: 63-310

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Drosophila, Hamster, Mouse, Rabbit, Rat, Xenopus, Chicken
Immunogen:
This MAP2K2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-229 amino acids from human MAP2K2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
44 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAP2K2
Additional Names:
Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK 2, MAP2K2, MEK2, MKK2, PRKMK2
Protein Accession Number:
P36507
Protein Gi Number:
547915
Ncbi Gene Id Number:
5605
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.
Background Reference 1:
Burroughs, K.D., et al., Mol. Cancer Res. 1(4):312-322 (2003).
Background Reference 2:
Tran, H., et al., Mol. Cell. Biol. 23(20):7177-7188 (2003).
Background Reference 3:
Li, S.P., et al., Cancer Res. 63(13):3473-3477 (2003).
Background Reference 4:
Li, Y., et al., J. Biol. Chem. 278(16):13663-13671 (2003).

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-310

List Size:
400 ul

List Price:

$495.00

Datasheet
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