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43-442 (0.3ug/ml) staining of Daudi (A), Jurkat (B), K562 (C) and Molt4 (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-442 (0.3ug/ml) staining of Daudi (A), Jurkat (B), K562 (C) and Molt4 (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MAP2K2 / MEK2 Antibody

CATALOG NUMBER: 43-442

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-DLKMLTNHTFIKRS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 48kDa band observed in lysates of cell lines Daudi, Jurkat, K562 and Molt4 (calculated MW of 44.4kDa according to NP_109587.1). Recommended concentration: 0.1-0.3ug/ml.
Predicted Molecular Weight:
Approx 48kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MAP2K2 / MEK2
Additional Names:
MAP2K2, mitogen-activated protein kinase kinase 2, MAPKK2, MEK2, MKK2, PRKMK2, ERK activator kinase 2, MAP kinase kinase 2, MAPK/ERK kinase 2, dual specificity mitogen-activated protein kinase kinase 2, mitogen-activated protein kinase kinase 2, p45
Protein Accession Number:
NP_109587.1
Protein Gi Number:
13489054
Ncbi Gene Id Number:
5605
Background Reference 1:
Duhamel S, Hébert J, Gaboury L, Bouchard A, Simon R, Sauter G, Basik M, Meloche S.Sef downregulation by Ras causes MEK1/2 to become aberrantly nuclear localized leading to polyploidy and neoplastic transformation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-442

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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