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Western blot analysis of extracts of various cell lines, using MAP2K4 antibody (23-132) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human kidney using MAP2K4 antibody (23-132) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using MAP2K4 antibody (23-132) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug MAP2K4 antibody (23-132). Western blot was performed from the immunoprecipitate using MAP2K4 antibody (23-132) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using MAP2K4 antibody (23-132) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

MAP2K4 Antibody

CATALOG NUMBER: 23-132

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide of human MAP2K4
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:1000 - 1:4000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:100
Positive Control 1:
BT-474
Positive Control 2:
MCF7
Positive Control 3:
SH-SY5Y
Positive Control 4:
Rat skeletal muscle
Predicted Molecular Weight:
Observed: 44kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MAP2K4
Additional Names:
MAP2K4, JNK activating kinase 1, JNK-activated kinase 1, JNK-activating kinase 1, MAPK/ERK kinase 4, MAPKK4, JNKK1, PRKMK4, SAPKK1, SEK1, MEK4, MKK4, SAPK/ERK kinase 1, SERK1, JNKK, MAP kinase kinase 4, MAPKK 4, MEK 4, SAPK kinase 1, SAPKK-1, SKK1
Ncbi Gene Id Number:
6416
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-132

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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