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Western blot analysis of extracts of various cell lines, using MAP2K5 antibody (22-583) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
Immunohistochemistry of paraffin-embedded rat spinal cord using MAP2K5 Antibody (22-583) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using MAP2K5 Antibody (22-583) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using MAP2K5 Antibody (22-583) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using MAP2K5 Antibody (22-583) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using MAP2K5 antibody (22-583). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using MAP2K5 antibody (22-583) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.

MAP2K5 Antibody

CATALOG NUMBER: 22-583

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human MAP2K5 (NP_660143.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Positive Control 1:
NCI-H460
Positive Control 2:
BT-474
Positive Control 3:
Mouse liver
Positive Control 4:
Rat liver
Positive Control 5:
Rat kidney
Predicted Molecular Weight:
Observed: 50kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MAP2K5
Additional Names:
Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MAPK/ERK kinase 5, MEK 5, MAP2K5, MEK5, MKK5, PRKMK5
Ncbi Gene Id Number:
5607
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-583

List Size:
100 uL

List Price:

$436.00

Datasheet
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