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Western blot analysis of MAP2K5 using rabbit polyclonal MAP2K5 Antibody (S149).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K5 gene (Lane 2).
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K5 Antibody (S149) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of MAP2K5 using rabbit polyclonal MAP2K5 Antibody (S149).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K5 gene (Lane 2).

MAP2K5 Antibody

CATALOG NUMBER: 63-281

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MAP2K5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-156 amino acids from human MAP2K5.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAP2K5
Additional Names:
Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MAPK/ERK kinase 5, MEK 5, MAP2K5, MEK5, MKK5, PRKMK5
Protein Accession Number:
Q13163
Protein Gi Number:
118572669
Ncbi Gene Id Number:
5607
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MAP2K5 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. It specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by MAP2K5 is involved in growth factor stimulated cell proliferation and muscle cell differentiation.
Background Reference 1:
Raviv,Z.,J. Cell. Sci. 117 (PT 9), 1773-1784 (2004)
Background Reference 2:
Kato,Y.,EMBO J. 16 (23), 7054-7066 (1997)
Background Reference 3:
English,J.M.,J. Biol. Chem. 274 (44), 31588-31592 (1999)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-281

List Size:
400 ul

List Price:

$495.00

Datasheet
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