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Western blot analysis of extracts from 3T3 cells treated with insulin using MAP2K7 (Phospho-Ser271) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded humanbrain tissue, using MAP2K7 (Phospho-Ser271) antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from 3T3 cells treated with insulin using MAP2K7 (Phospho-Ser271) Antibody. The lane on the right is treated with the antigen-specific peptide.

MAP2K7 (phospho Ser271) Antibody

CATALOG NUMBER: 79-855

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
MAP2K7 (phospho Ser271) antibody was raised against a peptide sequence around phosphorylation site of Serine271 (V-D-S (p)-K-A) derived from Human MAP2K7.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
The antibody detects endogenous levels of MAP2K7 only when phosphorylated at serine 271.
Predicted Molecular Weight:
60 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
MAP2K7
Additional Names:
PRKMK7, JNKK2, MKK7, MAP2K7, MP2K7
Protein Accession Number:
NP_660186.1
Protein Gi Number:
21735544
Ncbi Gene Id Number:
5609
Background:
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined.
Background Reference 1:
Wu Z., Mol. Cell. Biol. 17:7407-7416(1997).
Background Reference 2:
Lu X., J. Biol. Chem. 272:24751-24754(1997).
Background Reference 3:
Foltz I.N., J. Biol. Chem. 273:9344-9351(1998).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-855

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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