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Western Blot at 1:1000 dilution + human fetal brain lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
MAP3K15 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + human fetal brain lysate Lysates/proteins at 20 ug per lane.

MAP3K15 Antibody

CATALOG NUMBER: 56-348

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This MAP3K15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-342 amino acids from the N-terminal region of human MAP3K15.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
147 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAP3K15
Additional Names:
Mitogen-activated protein kinase kinase kinase 15, Apoptosis signal-regulating kinase 3, MAPK/ERK kinase kinase 15, MEK kinase 15, MEKK 15, MAP3K15, ASK3
Protein Accession Number:
Q6ZN16
Protein Gi Number:
116248533
Ncbi Gene Id Number:
389840
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of themitogen-activated protein kinase (MAPK) family. These familymembers function in a protein kinase signal transduction cascade,where an activated MAPK kinase kinase (MAP3K) phosphorylates andactivates a specific MAPK kinase (MAP2K), which then activates aspecific MAPK. This MAP3K protein plays an essential role inapoptotic cell death triggered by cellular stresses. [provided byRefSeq].
Background Reference 1:
Kaji, T., et al. Biochem. Biophys. Res. Commun. 395(2):213-218(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-348

List Size:
400 ul

List Price:

$495.00

Datasheet
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