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Western Blot at 1:1000 dilution + Raji whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution + Raji whole cell lysate Lysates/proteins at 20 ug per lane.

MAP4K1 Antibody

CATALOG NUMBER: 63-315

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MAP4K1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from human MAP4K1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
91 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAP4K1
Additional Names:
Mitogen-activated protein kinase kinase kinase kinase 1, Hematopoietic progenitor kinase, MAPK/ERK kinase kinase kinase 1, MEK kinase kinase 1, MEKKK 1, MAP4K1, HPK1
Protein Accession Number:
Q92918
Protein Gi Number:
29427916
Ncbi Gene Id Number:
11184
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MAP4K1 or HPKI (hematopoietic progenitor kinase I) is one of these mammalian kinases that have significant sequence similarity to the Saccharomyces Cerevisiae serine/threonine kinase STE20, which relays signals from G protein coupled receptors to cytosolic MAP kinase cascades. MAP4K1 may play a role in the response to environmental stress. It appears to act upstream of the JUN N terminal pathway. It may play a role in hematopoietic lineage decisions and growth regulation.
Background Reference 1:
Hu M.C.-T., Genes Dev. 10:2251-2264(1996).
Background Reference 2:
Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Background Reference 3:
Wissing J., Mol. Cell. Proteomics 6:537-547(2007).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-315

List Size:
400 ul

List Price:

$495.00

Datasheet
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