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Western blot analysis of extracts from JK cells using MAPK1/3 (Phospho-Tyr205/222) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from JK cells using MAPK1/3 (Phospho-Tyr205/222) Antibody. The lane on the right is treated with the antigen-specific peptide.

MAPK1/3 (phospho Tyr205/222) Antibody

CATALOG NUMBER: 79-934

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
MAPK1/3 (phospho Tyr205/222) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 205/222 (K-G-Y (p)-T-K) derived from Human MAPK1/3 .
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous levels of MAPK1/3 only when phosphorylated at tyrosine 205/222.
Predicted Molecular Weight:
44 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
MAPK1, MAPK3
Additional Names:
ERK, ERT1, MAPK 1, MAPK2, ERK2
Protein Accession Number:
NP_002736.3
Protein Gi Number:
66932916
Ncbi Gene Id Number:
5594;5595
Background:
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Background Reference 1:
Owaki H., Biochem. Biophys. Res. Commun. 182:1416-1422(1992).
Background Reference 2:
Dunham I., Nature 402:489-495(1999).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-934

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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