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Western blot analysis of anti-ERK2 C-term Pab in UV-treated HL-60 cell lysate
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ERK2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-ERK2 C-term Pab in UV-treated HL-60 cell lysate

MAPK1 Antibody

CATALOG NUMBER: 62-952

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Bovine
Immunogen:
This MAPK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-285 amino acids from the C-terminal region of human MAPK1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
41 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAPK1
Additional Names:
Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2, MAPK1, ERK2, PRKM1, PRKM2
Protein Accession Number:
P28482
Protein Gi Number:
119554
Ncbi Gene Id Number:
5594
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ERK2 is a member of the MAP kinase subfamily of Ser/Thr protein kinases. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.
Background Reference 1:
Grey, A., et al., Endocrinology 144(11):4886-4893 (2003).
Background Reference 2:
Sawafuji, K., et al., Am. J. Hematol. 72(1):67-69 (2003).
Background Reference 3:
Liao, Y., et al., Mol. Cell. Biol. 23(19):6836-6848 (2003).
Background Reference 4:
Garcia-Lora, A., et al., Cancer Immunol. Immunother. 52(1):59-64 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-952

List Size:
400 ul

List Price:

$495.00

Datasheet
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