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Western blot analysis of anti-P38gamma C-term Pab in mouse muscle tissue lysate
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with the P38 gamma antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-P38gamma C-term Pab in mouse muscle tissue lysate

MAPK12 Antibody

CATALOG NUMBER: 62-961

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This MAPK12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-367 amino acids from the C-terminal region of human MAPK12.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAPK12
Additional Names:
Mitogen-activated protein kinase 12, MAP kinase 12, MAPK 12, Extracellular signal-regulated kinase 6, ERK-6, Mitogen-activated protein kinase p38 gamma, MAP kinase p38 gamma, Stress-activated protein kinase 3, MAPK12, ERK6, SAPK3
Protein Accession Number:
P53778
Protein Gi Number:
2851522
Ncbi Gene Id Number:
6300
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
P38 gamma is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. P38 gamma functions as a signal transducer during differentiation of myoblasts to myotubes.
Background Reference 1:
Garcia-Lora, A., et al., Cancer Immunol. Immunother. 52(1):59-64 (2003).
Background Reference 2:
Julien, C., et al., J. Biol. Chem. 278(43):42615-42624 (2003).
Background Reference 3:
Robinson, M.J., et al., J. Biol. Chem. 277(7):5094-5100 (2002).
Background Reference 4:
Court, N.W., et al., J. Mol. Cell. Cardiol. 34(4):413-426 (2002).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-961

List Size:
400 ul

List Price:

$495.00

Datasheet
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