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Antibody is used in Western blot to detect P38 delta in nocodazole-treated HCT116 (lane 1) and PMA-treated Pam212 (lane 2) cell lysates.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with P38 delta antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Antibody is used in Western blot to detect P38 delta in nocodazole-treated HCT116 (lane 1) and PMA-treated Pam212 (lane 2) cell lysates.

MAPK13 Antibody

CATALOG NUMBER: 62-960

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This MAPK13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335-365 amino acids from the C-terminal region of human MAPK13.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAPK13
Additional Names:
Mitogen-activated protein kinase 13, MAP kinase 13, MAPK 13, Mitogen-activated protein kinase p38 delta, MAP kinase p38 delta, Stress-activated protein kinase 4, MAPK13, PRKM13, SAPK4
Protein Accession Number:
O15264
Ncbi Gene Id Number:
5603
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
P38 delta is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and cellular stress. MAP kinase kinases 3, and 6 can phosphorylate and activate this kinase. Transcription factor ATF2, and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase.
Background Reference 1:
Duyndam, M.C., et al., J. Biol. Chem. 278(9):6885-6895 (2003). Efimova, T., et al., J. Biol. Chem. 277(35):31753-31760 (2002). Knebel, A., et al., EMBO J. 20(16):4360-4369 (2001). Hu, M.C., et al., J. Biol. Chem. 274(11):7095-7102 (1999). Parker, C.G., et al., Biochem. Biophys. Res. Commun. 249(3):791-796 (1998).

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-960

List Size:
400 ul

List Price:

$495.00

Datasheet
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