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Western blot analysis of ERK8 using rabbit polyclonal ERK8 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK15 gene (Lane 2).
Western blot analysis of anti-ERK8 Antibody in T47D cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ERK8 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of ERK8 using rabbit polyclonal ERK8 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK15 gene (Lane 2).

MAPK15 Antibody

CATALOG NUMBER: 62-984

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MAPK15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human MAPK15.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
60 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAPK15
Additional Names:
Mitogen-activated protein kinase 15, MAP kinase 15, MAPK 15, Extracellular signal-regulated kinase 7, ERK-7, Extracellular signal-regulated kinase 8, ERK-8, MAPK15, ERK7, ERK8
Protein Accession Number:
Q8TD08
Protein Gi Number:
74760462
Ncbi Gene Id Number:
225689
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The ERKs are a subfamily of the MAPKs that have been implicated in cell growth and differentiation. Extracellular signal-regulated kinase 8 (Erk8) is a large MAP kinase whose activity is controlled by serum and the c-Src non-receptor tyrosine kinase. ERK8 down-regulates transactivation of the glucocorticoid receptor through Hic-5 and can negatively regulate transcriptional co-activation of androgen receptor and GRalpha by Hic-5 in a kinase-independent manner, suggesting a broader role for ERK8 in the regulation of nuclear receptors beyond estrogen receptor alpha. Erk8 is a novel effector of RET/PTC3 and, therefore, RET biological functions.
Background Reference 1:
Saelzler,M.P., J. Biol. Chem. 281 (24), 16821-16832 (2006)
Background Reference 2:
Iavarone,C., J. Biol. Chem. 281 (15), 10567-10576 (2006)
Background Reference 3:
Klevernic,I.V., Biochem. J. 394 (PT 1), 365-373 (2006)
Background Reference 4:
Suzuki,Y., Genome Res. 14 (9), 1711-1718 (2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-984

List Size:
400 ul

List Price:

$495.00

Datasheet
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