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45-087 (0.3ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-087 (0.3ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

MAPK3 Antibody

CATALOG NUMBER: 45-087

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: GGEPRRTEGVGP-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 45kDa band observed in HEK293 lysates (calculated MW of 43.1kDa according to NP_002737.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
Specificity:
No cross-reactivity expected with ERK2
Positive Control 1:
Cat. No. 1210 - 293 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MAPK3
Additional Names:
MAPK3, ERK1, mitogen-activated protein kinase 3, HGNC:6877, P44ERK1, P44MAPK, PRKM3, protein kinase, mitogen-activated 3 (MAP kinase 3 p44), HS44KDAP, HUMKER1A, MGC20180, OTTHUMP00000174538, extracellular signal-regulated kinase 1, extracellular signal-related kinase 1
Protein Accession Number:
NP_002737.2,NP_001035145.1,NP_001103361.1
Protein Gi Number:
38257141
Ncbi Gene Id Number:
5595
Background Reference 1:
Wang PY, Weng J, Anderson RG. OSBP is a cholesterol-regulated scaffolding protein in control of ERK 1/2 activation. Science. 2005 Mar 4;307(5714):1472-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-087

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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