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Western blot analysis of lysate from Hela cell line, using JNK1 antibody at 1:1000 at each lane.
JNK1-T183/Y185 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LJNK1-T183/Y185 antibody for immunohistochemistry.
Western blot analysis of lysate from Hela cell line, using JNK1 antibody at 1:1000 at each lane.

MAPK8 Antibody

CATALOG NUMBER: 55-516

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Xenopus
Immunogen:
This MAPK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-195 amino acids from human MAPK8.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
48 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAPK8
Additional Names:
Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, SAPK1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, MAPK8, JNK1, PRKM8, SAPK1, SAPK1C
Protein Accession Number:
P45983
Protein Gi Number:
2507195
Ncbi Gene Id Number:
5599
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the MAPkinase family. MAP kinases act as an integration point for multiplebiochemical signals, and are involved in a wide variety of cellularprocesses such as proliferation, differentiation, transcriptionregulation and development. This kinase is activated by variouscell stimuli, and targets specific transcription factors, and thusmediates immediate-early gene expression in response to cellstimuli. The activation of this kinase by tumor-necrosis factoralpha (TNF-alpha) is found to be required for TNF-alpha inducedapoptosis. This kinase is also involved in UV radiation inducedapoptosis, which is thought to be related to cytochrom c-mediatedcell death pathway. Studies of the mouse counterpart of this genesuggested that this kinase play a key role in T cell proliferation,apoptosis and differentiation. Four alternatively splicedtranscript variants encoding distinct isoforms have been reported.
Background Reference 1:
Dasgupta, J., et al. J. Cell. Physiol. 225(1):52-62(2010)
Background Reference 2:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 3:
Gao, D., et al. J. Biol. Chem. 285(39):29965-29973(2010)
Background Reference 4:
Han, J.S., et al. Anticancer Res. 30(9):3407-3412(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-516

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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