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Western blot analysis of anti-JNK1 Pab in HL60 (UV-treated) cell lysate
Confocal immunofluorescent analysis of MAPK8 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
MAPK8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of anti-JNK1 Pab in HL60 (UV-treated) cell lysate

MAPK8 Antibody

CATALOG NUMBER: 62-956

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This MAPK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-389 amino acids from the C-terminal region of human MAPK8.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
48 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAPK8
Additional Names:
Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, SAPK1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, MAPK8, JNK1, PRKM8, SAPK1, SAPK1C
Protein Accession Number:
P45983
Protein Gi Number:
2507195
Ncbi Gene Id Number:
5599
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
JNK1 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation.
Background Reference 1:
Deng, Y., et al., Cell 115(1):61-70 (2003).
Background Reference 2:
Luo, W., et al., J. Biol. Chem. 278(39):37451-37458 (2003).
Background Reference 3:
Ghosh, J., Biochem. Biophys. Res. Commun. 307(2):342-349 (2003).
Background Reference 4:
Utsugi, M., et al., J. Immunol. 171(2):628-635 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-956

List Size:
400 ul

List Price:

$495.00

Datasheet
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