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Western blot analysis of extracts from RAW264.7 cells treated with UV using MAPKAPK2 (Phospho-Ser272) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAPKAPK2 (Phospho-Ser272) antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from RAW264.7 cells treated with UV using MAPKAPK2 (Phospho-Ser272) Antibody. The lane on the right is treated with the antigen-specific peptide.

MAPKAPK2 (phospho Ser272) Antibody

CATALOG NUMBER: 79-915

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
MAPKAPK2 (phospho Ser272) antibody was raised against a peptide sequence around phosphorylation site of Serine 272 (A-I-S (p)-P-G) derived from Human MAPKAPK2.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
The antibody detects endogenous levels of MAPKAPK2 only when phosphorylated at serine 272.
Predicted Molecular Weight:
45 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
MAPKAPK2
Additional Names:
MAPK2; , MAPKAPK2, RPS6KC1
Protein Accession Number:
NP_116584.2
Protein Gi Number:
32481209
Ncbi Gene Id Number:
9261
Background:
This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.
Background Reference 1:
Zu Y.-L., Biochem. Biophys. Res. Commun. 200-1118-1124(1994).
Background Reference 2:
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Background Reference 3:
Stokoe D., Biochem. J. 296:843-849(1993).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-915

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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