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Western blot analysis in mouse brain tissue lysates (15ug/lane).This demonstrates the detected MAPRE1 protein (arrow).
MAPRE1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse brain tissue lysates (15ug/lane).This demonstrates the detected MAPRE1 protein (arrow).

MAPRE1 Antibody

CATALOG NUMBER: 62-452

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Rat, Xenopus
Immunogen:
This MAPRE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-71 amino acids from the N-terminal region of human MAPRE1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
30 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAPRE1
Additional Names:
Microtubule-associated protein RP/EB family member 1, APC-binding protein EB1, End-binding protein 1, EB1, MAPRE1
Protein Accession Number:
Q15691
Protein Gi Number:
20138589
Ncbi Gene Id Number:
22919
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene was first identified byits binding to the APC protein which is often mutated in familialand sporadic forms of colorectal cancer. This protein localizes tomicrotubules, especially the growing ends, in interphase cells.During mitosis, the protein is associated with the centrosomes andspindle microtubules. The protein also associates with componentsof the dynactin complex and the intermediate chain of cytoplasmicdynein. Because of these associations, it is thought that thisprotein is involved in the regulation of microtubule structures andchromosome stability. This gene is a member of the RP/EB family.
Background Reference 1:
Jaulin, F., et al. J. Cell Biol. 190(3):443-460(2010)
Background Reference 2:
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
Background Reference 3:
De Groot, C.O., et al. J. Biol. Chem. 285(8):5802-5814(2010)
Background Reference 4:
Jiang, K., et al. EMBO Rep. 10(8):857-865(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-452

List Size:
400 ul

List Price:

$495.00

Datasheet
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