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Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in IHC on mouse cortex at: 1:1000.
Antibody used in WB on N2A at 1:2000 (Lane 1: 10ug N2A expressed human Tau-C Lane 2: 10ug N2A expressed human Tau-E Lane 3: 10ug N2A expressed human Tau-F Lane 4: 10ug N2A expressed human Tau-B Lane 5: 10ug N2A expressed human Fetal-Tau Lane 6: 10ug N2A expressed human Tau-D Lane 7: 10ug N2A expressed human much Fetal-Tau Lane 8: 10ug N2A lysate ).
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.

MAPT Antibody

CATALOG NUMBER: 30-353

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MAPT.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
MAPT antibody can be used for detection of MAPT by ELISA at 1:312500. MAPT antibody can be used for detection of MAPT by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
46 kDa, 40 kDa, 79 kDa, 37 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store MAPT antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
MAPT
Additional Names:
MAPT, DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU
Protein Accession Number:
NP_005901
Protein Gi Number:
6754638
Ncbi Gene Id Number:
4137
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MAPT is differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. The mutations in the gene have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
Background Reference 1:
Reynolds, M.R., (2006) Biochemistry 45 (13), 4314-4326.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-353

List Size:
100 ul

List Price:

$519.00

Datasheet
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