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46-938 (0.3ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-938 (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-938 (0.3ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

MARCH10 Antibody

CATALOG NUMBER: 46-938

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Rat
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-SRNYPQPRTEE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 90 kDa band observed in Rat Testis lysates (calculated MW of 90.5 kDa according to Human NP_689811.2 and of 87.9 kDa according to Mouse NP_766156.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms (NP_001094345.1; NP_689811.2), which are identical.
Positive Control 1:
Cat. No. 1476 - Rat Testis Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
40238
Additional Names:
MARCH10, membrane-associated ring finger (C3HC4) 10 , FLJ35757, MARCH-X, RNF190, ring finger protein 190
Protein Accession Number:
NP_001094345.1,NP_689811.2
Protein Gi Number:
154937342
Ncbi Gene Id Number:
162333
Background Reference 1:
Morokuma Y, Nakamura N, Kato A, Notoya M, Yamamoto Y, Sakai Y, Fukuda H.Yamashina S, Hirata Y, Hirose S. MARCH-XI, a novel transmembrane ubiquitin ligase implicated inubiquitin-dependent protein sorting in developing spermatids. J Biol Chem. 2007 Aug 24;282(34):24806-15. Epub 2007 Jun 29.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-938

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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