MARCH2 Antibody

CATALOG NUMBER: 25-810

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MARCH2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
MARCH2 antibody can be used for detection of 40239 by ELISA at 1:312500. MARCH2 antibody can be used for detection of 40239 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
27 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store MARCH2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
MARCH2
Additional Names:
MARCH2, HSPC240, MARCH-II, RNF172
Protein Accession Number:
NP_001005415
Protein Gi Number:
53729332
Ncbi Gene Id Number:
51257
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MARCH2 is an E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC and CD86, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. MARCH2 may be involved in endosomal trafficking through interaction with STX6.
Background Reference 1:
Cao, Z., (2008) Cell. Signal. 20 (1), 73-82.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-810

List Size:
100 ul

List Price:

$519.00

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