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Antibody used in WB on Human HeLa at 0.2-1 ug/ml.
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.

MARCH4 Antibody

CATALOG NUMBER: 25-846

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MARCH4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
MARCH4 antibody can be used for detection of 40241 by ELISA at 1:312500. MARCH4 antibody can be used for detection of 40241 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight:
45 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store MARCH4 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
MARCH4
Additional Names:
MARCH4, MARCH-IV, MGC104908, RNF174
Protein Accession Number:
NP_065865
Protein Gi Number:
57977325
Ncbi Gene Id Number:
57574
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MARCH4 is an E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Background Reference 1:
Bartee, E., (2004) J. Virol. 78 (3), 1109-1120.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-846

List Size:
100 ul

List Price:

$519.00

Datasheet
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