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Antibody used in WB on Human Liver at 0.2-1 ug/ml.
Antibody used in WB on Human Liver at 0.2-1 ug/ml.

MARCH5 Antibody

CATALOG NUMBER: 25-835

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MARCH5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
MARCH5 antibody can be used for detection of MARCH5 by ELISA at 1:1562500. MARCH5 antibody can be used for detection of MARCH5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10409 - Fetal Liver Tissue Lysate
Predicted Molecular Weight:
31 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store MARCH5 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
MARCH5
Additional Names:
MARCH5, FLJ20445, MARCH-V, MITOL, RNF153
Protein Accession Number:
NP_060294
Protein Gi Number:
8923415
Ncbi Gene Id Number:
54708
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MARCH5 is an ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2) and DRP1 (DNM1L).MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).
Background Reference 1:
Karbowski, M., (2007) J. Cell Biol. 178 (1), 71-84.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-835

List Size:
100 ul

List Price:

$519.00

Datasheet
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