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46-718 (0.2ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-718 (0.2ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MARCH6 Antibody

CATALOG NUMBER: 46-718

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: C-QGRRVIFQKVKE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 100kDa band observed in Human Lung lysates (calculated MW of 103kDa according to NP_005876.2). Recommended concentration: 0.2-0.5ug/ml.An additional band of unknown identity was also consistently observed at 18kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Positive Control 1:
Cat. No. 1302 - Human Lung Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
38777
Additional Names:
MARCH-VI, TEB4, MARCH6, membrane-associated ring finger (C3HC4) 6, KIAA0597, RNF176, membrane-associated RING-CH protein VI
Protein Accession Number:
NP_005876.2
Protein Gi Number:
33589846
Ncbi Gene Id Number:
10299
Background Reference 1:
Kreft SG, Wang L, Hochstrasser M. Membrane topology of the yeast endoplasmic reticulum-localized ubiquitin ligase Doa10 and comparison with its human ortholog TEB4 (MARCH-VI). J Biol Chem. 2006 Feb 24;281(8):4646-53. Epub 2005 Dec 22.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-718

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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