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Western blot analysis in T47D cell line lysates (35ug/lane).
Western blot analysis in T47D cell line lysates (35ug/lane).

MARCH7 Antibody

CATALOG NUMBER: 58-601

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This MARCH7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-95 amino acids from the N-terminal region of human MARCH7.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
78 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MARCH7
Additional Names:
E3 ubiquitin-protein ligase MARCH7, 632-, Axotrophin, Membrane-associated RING finger protein 7, Membrane-associated RING-CH protein VII, MARCH-VII, RING finger protein 177, MARCH7, AXOT, RNF177
Protein Accession Number:
Q9H992
Protein Gi Number:
74762745
Ncbi Gene Id Number:
64844
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MARCH7 is a member of the MARCH family of membrane-boundE3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin(see MIM 191339) to target lysines in substrate proteins, therebysignaling their vesicular transport between membrane compartments(Bartee et al., 2004 [PubMed 14722266]).
Background Reference 1:
Szigyarto, C.A., et al. J. Histochem. Cytochem. 58(4):301-308(2010)
Background Reference 2:
Nathan, J.A., et al. Traffic 9(7):1130-1145(2008)
Background Reference 3:
Muthukumarana, P.A., et al. Int. Immunopharmacol.(13-14), 1993-2001 (2006) :
Background Reference 4:
Flierman, D., et al. Proc. Natl. Acad. Sci. U.S.A. 103(31):11589-11594(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-601

List Size:
400 ul

List Price:

$495.00

Datasheet
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