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Western blot analysis of MARCH8 in HeLa cell lysate with MARCH8 antibody at (A) 0.5 μg/ml and (B) 1 μg/mL.
Immunocytochemistry of MARCH8 in HeLa cells with MARCH8 antibody at 2.5 μg/mL.
Western blot analysis of MARCH8 in HeLa cell lysate with MARCH8 antibody at (A) 0.5 μg/ml and (B) 1 μg/mL.
Trial Size

MARCH8 Antibody

CATALOG NUMBER: 4885

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (76%)
Immunogen:
MARCH8 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MARCH8.
The immunogen is located within the last 50 amino acids of MARCH8.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
MARCH8 antibody can be used for detection of MARCH8 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Purification:
MARCH8 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MARCH8 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MARCH8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MARCH8
Additional Names:
MARCH8 Antibody: MIR, c-MIR, RNF178, MARCH-VIII, MIR, E3 ubiquitin-protein ligase MARCH8, Cellular modulator of immune recognition
Protein Accession Number:
NP_659458
Protein Gi Number:
50539410
Ncbi Gene Id Number:
220972
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MARCH8 Antibody: MARCH8 (c-MIR) is a novel E3 ubiquitin ligase designated as the modulator of immune recognition (MIR) family, whose catalytic domain is a variant RING domain (RING-CH domain). MARCH8 was found as a functional and structural homolog of KSHV MIR1 and MIR2. MARCH8 targets B7-2 to lysosomal degradation and down-regulates the B7-2 surface expression through ubiquitination of its cytoplasmic tail. Furthermore, MARCH8 has been shown to down-regulate the expression of transferrin receptor and Fas, an important molecule for the induction of apoptosis. MARCH8 is the first example of an E3 ubiquitin ligase that is capable of inhibiting MHC II expression. Recent findings suggest that MARCH8 may regulate immune responses by promoting ubiquitination of MHC-II and CD86, leading to their subsequent endocytosis and lysosomal degradation.
Background Reference 1:
Coscoy L, Sanchez DJ, and Ganem D. A novel class of herpesvirus-encoded membrane-bound E3 ubiquitin ligases regulates endocytosis of proteins involved in immune recognition. J. Cell Biol. 2001; 155:126573.
Background Reference 2:
Goto E, Ishido S, Sato Y, et al. c-MIR, a human E3 ubiquitin ligase, is a functional homolog of herpesvirus proteins MIR1 and MIR2 and has similar activity. J. Biol. Chem.2003; 278:14657-68.
Background Reference 3:
Ohmura-Hoshino M, Goto E, Matsuki Y, et al. A novel family of membrane-bound E3 ubiquitin ligases. J. Biochem.2006; 140:147-54.
Background Reference 4:
Bartee E, Mansouri M, Hovey Nerenberg BT, et al. Downregulation of major histocompatibility complex class I by human ubiquitin ligases related to viral immune evasion proteins. J. Virol.2004; 78:1109-20.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4885

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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