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Western blot analysis in SK-BR-3 cell line lysates (35ug/lane).
Western blot analysis in SK-BR-3 cell line lysates (35ug/lane).

MARCH9 Antibody

CATALOG NUMBER: 59-150

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This MARCH9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-330 amino acids from the C-terminal region of human MARCH9.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
38 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MARCH2
Additional Names:
E3 ubiquitin-protein ligase MARCH9, 632-, Membrane-associated RING finger protein 9, Membrane-associated RING-CH protein IX, MARCH-IX, RING finger protein 179, MARCH9, RNF179
Protein Accession Number:
Q86YJ5
Protein Gi Number:
74759533
Ncbi Gene Id Number:
92979
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MARCH9 is a member of the MARCH family of membrane-boundE3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin(see MIM 191339) to target lysines in substrate proteins, therebysignaling their vesicular transport between membrane compartments.MARCH9 induces internalization of several membrane glycoproteinsand directs them to the endosomal compartment (Bartee et al., 2004[PubMed 14722266]; Hoer et al., 2007 [PubMed 17174307]).[suppliedby OMIM].
Background Reference 1:
Nice, T.J., et al. J. Immunol. 185(9):5369-5376(2010)
Background Reference 2:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 3:
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Background Reference 4:
Hoer, S., et al. FEBS Lett. 581(1):45-51(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-150

List Size:
400 ul

List Price:

$495.00

Datasheet
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