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Western blot analysis of hMARCKS-K254 in Hela cell line lysates (35ug/lane)
Western blot analysis of hMARCKS-K254 in Hela cell line lysates (35ug/lane)

MARCKS Antibody

CATALOG NUMBER: 61-411

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MARCKS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-271 amino acids from the C-terminal region of human MARCKS.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
32 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MARCKS
Additional Names:
Myristoylated alanine-rich C-kinase substrate, MARCKS, Protein kinase C substrate, 80 kDa protein, light chain, 80K-L protein, PKCSL, MARCKS, MACS, PRKCSL
Protein Accession Number:
P29966
Protein Gi Number:
76803798
Ncbi Gene Id Number:
4082
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MARCKS is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.
Background Reference 1:
Rauch, M.E., et al., J. Biol. Chem. 277(16):14068-14076 (2002).
Background Reference 2:
Aderem, A., Biochem. Soc. Trans. 23(3):587-591 (1995).
Background Reference 3:
Rao, P.H., et al., Cytogenet. Cell Genet. 66(4):272-273 (1994).
Background Reference 4:
Taniguchi, H., et al., J. Biol. Chem. 268(14):9960-9963 (1993).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-411

List Size:
400 ul

List Price:

$495.00

Datasheet
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