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Western blot of rat brain lysate showing phospho-specific immunolabeling of the ~87k MARCKS protein.
Western blot of rat brain lysate showing phospho-specific immunolabeling of the ~87k MARCKS protein.

MARCKS (phospho Ser152 / 156) Antibody

CATALOG NUMBER: XPS-1015

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Frog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Frog, Human, Mouse, Rat
Immunogen:
MARCKS (Ser152/156) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser152 and phospho-Ser156 of rat MARCKS.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Immunolabeling of the 87kDa band in rat brain homogenate is blocked by the Ser152/156 phosphopeptide but not by the non-phosphopeptide. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot Blots (DB) and Western Blot (WB). Suitability for Immunohistochemistry (IHC) not yet determined. Human, mouse, rat, and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Specificity:
MARCKS antibody is specific for MARCKS phosphorylated at Ser152 and Ser156.
Predicted Molecular Weight:
87
Purification:
Affinity Purified
Clonality:
Polyclonal
Physical State:
Liquid
Concentration:
batch dependent
Storage Conditions:
For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Ncbi Official Symbol:
Marcks
Protein Accession Number:
P30009
Protein Gi Number:
266495
Ncbi Gene Id Number:
25603
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Moreover, MARCKS is a member of a family of calmodulin binding proteins. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.
Background Reference 1:
Palmer, R.H., Schönwasser, D.C., Rahman, D., Pappin, D.J.C., Herget, T., and Parker, P.J., "PRK1 phosphorylates MARCKS at the PKC sites: Serine 152, serine 156 and serine 163," FEBS Letters, 378 (1996) 281 - 285.
Background Reference 2:
Spizz, G. and Blackshear, P.J., "Protein kinase C-mediated phosphorylation of the myristoylated alanine-rich C-kinase substrate protects it from specific proteolytic cleavage," J. Biol. Chem., 271 (1996) 553 - 562.
Background Reference 3:
Thelen, M., Rosen, A., Nairn, A.C., and Aderem, A., "Regulation by phosphorylation of reversible association of a myristoylated protein kinase C substrate with the plasma membrane," Nature (London), 351 (1991) 320 - 322.
Background Reference 4:
Hartwig, J.H., Thelen, M., Rosen, A., Janmey, P.A., Nairn, A.C., and Aderem, A., "MARCKS is an actin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin," Nature (London), 356 (1992) 618 - 622.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-1015

List Size:
0.1 mL

List Price:

$540.00

Datasheet
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